polyvinylidene fluoride pvdf membranes (Bio-Rad)

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Bio-Rad polyvinylidene fluoride pvdf membranes
Polyvinylidene Fluoride Pvdf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 21611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immun+blot+polyvinylidene+fluoride+membrane/Immun-Blot+PVDF+Membrane/pmc13129387-230-14-19
Average 96 stars, based on 21611 article reviews

polyvinylidene fluoride pvdf membranes - by Bioz Stars, 2026-09

96/100 stars

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Protease Inhibitor:

Article Title: AXL promotes inflammatory breast cancer progression by regulating immunosuppressive macrophage polarization.
Article Snippet: A PureLink RNA Mini Kit (Thermo Fisher Scientific; 12183018A), amfiRivert cDNA Synthesis Platinum Master Mix (GenDEPOT, Barker, TX; R5600), and iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA; 1725121) were used for quantitative reverse transcription-polymerase chain reaction (qRTPCR) analysis. .. A protease inhibitor and phosphatase inhibitor cocktail (Bimake.com, Houston, TX; B14001 and B15001), NuPAGE LDS Sample Buffer (Thermo Fisher Scientific; NP0007), 12% NuPAGE Bis–Tris gels (Thermo Fisher Scientific; NP0322), and an Immun-Blot polyvinylidene fluoride membrane (Bio-Rad; 1620177) were used for cell lysis, gel running, and membrane transfer. .. The following primary antibodies were used for Western blotting: anti-human phospho-AXL ([Y779] R&D Systems, Minneapolis, MN; AF2228), anti-human AXL (Sigma; WH0000558M1), anti-mouse AXL (R&D Systems; AF854), anti-human phospho-STAT6 ([Tyr641] Cell Signaling Technology, Danvers, MA; 56554S), antihuman STAT6 (Cell Signaling Technology; 9362S), anti-human CD206 (R&D Systems; MAB25341), and antihuman β-Actin (Sigma; A5316).

Article Title: AXL promotes inflammatory breast cancer progression by regulating immunosuppressive macrophage polarization
Article Snippet: A PureLink RNA Mini Kit (Thermo Fisher Scientific; 12183018A), amfiRivert cDNA Synthesis Platinum Master Mix (GenDEPOT, Barker, TX; R5600), and iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA; 1725121) were used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis. .. A protease inhibitor and phosphatase inhibitor cocktail (Bimake.com, Houston, TX; B14001 and B15001 ), NuPAGE LDS Sample Buffer (Thermo Fisher Scientific; NP0007), 12% NuPAGE Bis–Tris gels (Thermo Fisher Scientific; NP0322), and an Immun-Blot polyvinylidene fluoride membrane (Bio-Rad; 1620177) were used for cell lysis, gel running, and membrane transfer. .. The following primary antibodies were used for Western blotting: anti-human phospho-AXL ([Y779] R&D Systems, Minneapolis, MN; AF2228), anti-human AXL (Sigma; WH0000558M1), anti-mouse AXL (R&D Systems; AF854), anti-human phospho-STAT6 ([Tyr641] Cell Signaling Technology, Danvers, MA; 56554S), anti-human STAT6 (Cell Signaling Technology; 9362S), anti-human CD206 (R&D Systems; MAB25341), and anti-human β-Actin (Sigma; A5316).

Membrane:

Article Title: Oxidative stress-induced proteolytic activation of polyphenol oxidase triggers an oxidized flavonoids-mediated stress signaling in Camellia sinensis
Article Snippet: .. Equal amounts of proteins were separated on 10% SDS-PAGE and blotted onto Immun-Blot polyvinylidene fluoride membrane (Bio-Rad, USA). .. The PPO proteins were detected using rabbit anti-PPO (1:1000, catalog no. MBS9458498; MyBioSource, Inc.) and ECL prime western blotting detection reagent (Cytiva Amersham, Fisher scientific).

Article Title: FUS regulates RAN translation through modulating the G-quadruplex structure of GGGGCC repeat RNA in C9orf72 -linked ALS/FTD
Article Snippet: .. 5 μL of each supernatant were run on a 5–20% gradient polyacrylamide gel (Wako) and then transferred onto an Immun-Blot polyvinylidene fluoride membrane (Bio-Rad). .. Membranes were blocked with 5% skim milk in PBS containing 0.1% Tween-20 (PBST) or PVDF Blocking Reagent for Can Get Signal (TOYOBO) for 2 hr at RT, and then incubated overnight at 4°C with a rabbit polyclonal anti-FUS antibody (A300-302A, Bethyl Laboratories), a rat monoclonal anti-poly(GR) antibody (clone 5A2, MABN778, Millipore), a Living Colors EGFP mouse monoclonal antibody (632569, Clontech), or a mouse monoclonal anti-actin antibody (clone AC-40, A4700, Sigma-Aldrich) at 1:1000 dilution as the primary antibody.

Article Title: miR-204 Negatively Regulates HIV-Tat-Mediated Inflammation in Cervical Epithelial Cells via the NF-κB Axis: Insights from an In Vitro Study
Article Snippet: Bradford assay (Bio-Rad, Herculus, CA, USA) was used to determine the protein concentrations as per the manufacturer’s instructions. .. A total of 100 μg of soluble proteins was resolved in 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by blotting onto a 0.2 μm Immun-Blot polyvinylidene fluoride membrane (Bio-Rad, USA). ..

Article Title: miR-204 Negatively Regulates HIV-Tat-Mediated Inflammation in Cervical Epithelial Cells via the NF-κB Axis: Insights from an In Vitro Study
Article Snippet: Bradford assay (Bio-Rad, Herculus, CA, USA) was used to determine the protein concentrations as per the manufacturer’s instructions. .. A total of 100 μg of soluble proteins was resolved in 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE), followed by blotting onto a 0.2 μm Immun-Blot polyvinylidene fluoride membrane (Bio-Rad, USA). ..

Article Title: FUS regulates RAN translation through modulating the G-quadruplex structure of GGGGCC repeat RNA in C9orf72-linked ALS/FTD
Article Snippet: .. 5 μL of each supernatant were run on a 5–20% gradient polyacrylamide gel (Wako) and then transferred onto an Immun- Blot polyvinylidene fluoride membrane (Bio- Rad). .. Membranes were blocked with 5% skim milk in PBS containing 0.1% Tween- 20 (PBST) or PVDF Blocking Reagent for Can Get Signal (TOYOBO) for 2 hr at RT, and then incubated overnight at 4°C with a rabbit polyclonal anti- FUS antibody (A300- 302A, Bethyl Laboratories), a rat monoclonal anti- poly(GR) antibody (clone 5A2, MABN778, Millipore), a Living Colors EGFP mouse monoclonal antibody (632569, Clontech), or a mouse monoclonal anti- actin antibody (clone AC- 40, A4700, Sigma- Aldrich) at 1:1000 dilution as the primary antibody.

Article Title: Fragility of ER homeostatic regulation underlies haploid instability in human somatic cells
Article Snippet: .. Separated proteins were transferred onto an Immun-Blot polyvinylidene fluoride membrane (Bio-Rad). ..

Lysis:

SDS Page:

Nucleic Acid Electrophoresis:

Article Title: miR-204 Negatively Regulates HIV-Tat-Mediated Inflammation in Cervical Epithelial Cells via the NF-κB Axis: Insights from an In Vitro Study
Article Snippet: Bradford assay (Bio-Rad, Herculus, CA, USA) was used to determine the protein concentrations as per the manufacturer’s instructions. .. A total of 100 μg of soluble proteins was resolved in 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE), followed by blotting onto a 0.2 μm Immun-Blot polyvinylidene fluoride membrane (Bio-Rad, USA). ..

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